Supplementary Figure 2: Adenine editing efficiencies in mouse embryos.

(a) The target sequence at the Tyrosinase (Tyr) locus. The PAM sequence is shown in blue. The sgRNA target sequence is underlined. The targeted adenine in the wild-type sequence and the expected change in the sequence are shown in green and red, respectively. (b) Point mutation efficiencies associated with sgRNAs of different lengths (gX19, GX20, and GX21). Data are presented as mean ± s.e.m. (n = 8, 16 and 12 blastocysts, respectively). (c) Frequencies of blastocysts that carry the H420R mutation (Himalayan allele). (d) Alignments of mutant sequences from blastocysts after microinjection of ABE mRNA and Tyr-targeting sgRNAs of different lengths. The PAM and substitutions are shown in blue and red, respectively. The numbers in the column on the right indicate the frequency of each allele. WT, wild-type. H420R mutant allele frequencies are shown in red.