Supplementary Figure 6: Whole genome sequencing to assess off-target effects in the Tyr mutant mouse. | Nature Biotechnology

Supplementary Figure 6: Whole genome sequencing to assess off-target effects in the Tyr mutant mouse.

From: Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy

Supplementary Figure 6

Genomes from the Tyr mutant mouse (Tyr #4) and a wild-type (WT) control mouse were sequenced using Illumina HiSeq X10. We identified unique single nucleotide variants (SNVs) in Tyr #4 by trimming out those in the WT control using the program 'Strelka' with the default 'eland' option. None of these SNVs other than the variations at the on-target site were found at potential off-target sites, identified using Cas-OFFinder, with up to 7 mismatches (207,848 sites) or with up to 5 mismatches and a DNA or RNA bulge (1,030,669 sites).

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