Supplementary Figure 7: Growth curves of RTΔA cells containing point mutants in oxidative stress and selenite resistance.
From: Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains
Growth curves of RTΔA cells containing point mutants in oxidative stress and selenite resistance genes in rich and defined media ± 1 μM Na2SeO3. The curves are plotted with a line showing the mean and shading representing ± S.E.M. where n = three independent biological replicates. (a) Growth curves of RTΔA containing a constitutively active variant of OxyR (A233T) observed during evolution. The A233T mutation does not provide any clear benefits to cell fitness compared to a wild-type control. (b-d) Growth curves of RTΔA containing T69I, T73A or H153Y mutations respectively in CysK, which are expected to strongly inhibit cysteine biosynthesis, compared to a wild-type control. Mutations are not observed to have a significant impact on cell growth.
