Supplementary Figure 10: Mass spectrometry of GFP X177 expressed in β_CC1 and T_CC1 cells in the absence of Na2SeO3 confirms the incorporation of serine.
From: Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains
(a) Mass spectrum of intact GFP X177 expressed in β_CC1 cells. (b) Deconvoluted mass spectrum of GFP X177 expressed in β_CC1 cells reveals two species resulting from partial loss of the N-terminal methionine residue. (c) Experimentally determined monoisotopic masses (27887.87 Da and 27756.84 Da) match the theoretical masses of (27887.83 Da and 27756.79 Da) of GFP containing a serine residue at position 177. (d) Mass spectrum of intact GFP X177 expressed in T_CC1 cells. (e) Deconvoluted mass spectrum of GFP X177 expressed in T_CC1 cells. (f) Experimentally determined monoisotopic masses (27887.85 Da and 27756.84 Da) match the theoretical masses of (27887.83 Da and 27756.79 Da) of GFP containing a serine residue at position 177. All spectra are representative from a minimum of two technical replicates.
