Supplementary Figure 3: Growth curves in selenium free media (MOPS EZ) of parental strains and descendant populations evolved under β-lactam stress.
From: Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains
The labels indicate the media additives and the curves are plotted with a line showing the mean and shading representing ± S.E.M. where n = three independent biological replicates. Carbenicillin and Na2SeO3 were added at concentrations of 100 μg.mL and 1 μM respectively. (a) Δ parental strain and evolved populations β_Δ1-3. (b) CC parental strain and evolved populations β_CC1-3. (c) UC parental strain and evolved populations β_UC1-3. (d) UU parental strain and evolved populations β_UU1-3. The Δ and UU parental strains had severely impaired growth in MOPS EZ media, particularly when grown in 96-well plates. Evolved populations have increased growth rate and reach higher culture density compared to parental strains. All β_UC and β_UU populations retained selenocysteine dependence, requiring selenite supplementation to grow in the presence of carbenicillin.
