Supplementary Figure 5: Genotyping of GFP-deficient progenitor cells. | Nature Biotechnology

Supplementary Figure 5: Genotyping of GFP-deficient progenitor cells.

From: Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

Supplementary Figure 5

(A) Positions of primer pairs and the gRNA. Display conventions as in Fig. 3A. (B) Agarose gel pictures. Asterisks indicate clones with at least one deletion allele. Red asterisks indicate eight clones, whose deletion alleles were verified by Sanger sequencing (Fig. S6A). Ladder scale is in kilobases.

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