Supplementary Figure 7: FNLS increases target base editing, the ratio of desired vs non-desired editing
From: Optimized base editors enable efficient editing in cells, organoids and mice
a. Frequency (%) of C>T conversion in NIH/3T3 cells transduced with RA- or FNLS-P2A-Puro lentiviral vectors 2 days following introduction of different sgRNAs, as indicated. Editing in BE3-PGK-Puro cells (from Figure 1e) is shown for comparison. Graphs show mean values. Error bars represent s.e.m., n = 3 biologically independent experiments; asterisks (*) indicate a significant difference (p<0.05) between groups, using a two-way ANOVA with Tukey’s correction for multiple testing. b. Frequency (%) of unwanted target modifications (indels, C>A, C>G) in RA and FNLS expressing 3T3 cells generated with the P2A-Puro lentiviral vector. Graphs shows mean values +/- s.e.m.; n=3 biologically independent experiments. c. Relative change in base editing in FNLS-expressing lines, compared to RA cells. Graphs show mean values. Error bars represent s.e.m., n = 12 target cytosines across 5 different sgRNAs, includes day 2 and day 6; asterisks (*) indicate a significant difference (p<0.05) between groups, using an ANOVA with Tukey’s correction for multiple testing.
