Supplementary Figure 10: Editing at off target sites
From: Optimized base editors enable efficient editing in cells, organoids and mice
a. Frequency (%) of C>T conversion of any C in the editing window at two predicted off target sites for FANCF.S1 and CTNNB1.S45 in DLD1 cells expressing BE3, RA, or FNLS. Graph shows mean values. Error bars represent s.e.m., n = 3 biologically independent experiments. b. Sanger sequencing chromatograms showing detectable off target editing for the Apc.492 sgRNA (indicated by blue arrowheads) in NIH/3T3 cells. No editing was detected for either of two predicted off-target sites for Apc.1405, or the top predicted off-target site for Pik3ca.545. We were unable to amplify the Pik3ca_OT2 target region from genomic DNA. Bases highlighted green represent the target cytosine, while bases in black represent mismatches to the perfect sgRNA target site. Chromatograms are representative of three independent experiments, each with similar results.
