Supplementary Figure 11: 2X increases the range of editing in human and mouse cells
From: Optimized base editors enable efficient editing in cells, organoids and mice
a. Frequency (%) of C>T conversion in NIH/3T3 cells transduced with RA- or FNLS-P2A-Puro lentiviral vectors 2 and 6 days following introduction of different sgRNAs, as indicated. Editing in BE3-PGK-Puro cells (from Figure 1e) is shown for comparison. Graphs show mean values. Error bars represent s.e.m., n = 3 biologically independent experiments; asterisks (*) indicate a significant difference (p<0.05) between groups, using a two-way ANOVA with Tukey’s correction for multiple testing. b. Frequency (%) of unwanted target modifications (indels, C>A, C>G) in RA or 2X expressing NIH/3T3 cells at Day 6. Graph shows mean values. Error bars represent s.e.m., n=3 biologically independent experiments. c and d. Frequency (%) of target C>T conversion in DLD1 cells expressing either BE3, RA, or 2X, and infected with sgRNAs targeting FANCF.S1 (c) or CTNNB1.S45 (d). e. Frequency (%) of target C>T conversion in NIH/3T3 cells expressing either BE3, BE3RA, or 2X, and infected with an sgRNA targeting (mouse) Ctnnb1.S45. Graphs show mean values. Error bars represent s.e.m., n = 3 biologically independent experiments; asterisks (*) indicate a significant difference (p<0.05) between groups, using a two-way ANOVA with Tukey’s correction for multiple testing.
