Supplementary Figure 3: RA increases target base editing in transfection assays and improves the ratio of desired to non-desired target editing
From: Optimized base editors enable efficient editing in cells, organoids and mice
Frequency (%) of C>T conversion and indel formation in co-transfected HEK293T cells with BE3 or RA, and FANCF.S1 (a) or CTNNB1.S45 (b) sgRNAs. Graphs show mean values. Error bars indicate s.e.m., n = 4 biologically independent experiments, asterisks (*) indicate a significant difference (p<0.05) between groups, using a two-way ANOVA with Sidak’s correction for multiple testing. c. Frequency (%) of unwanted target modifications (indels, C>A, C>G) in BE3 or RA expressing 3T3 cells generated with the PGK-Puro lentiviral vector. Graph shows mean values +/- s.e.m., n=3 biologically independent experiments. d. Relative increase in target base editing in RA-expressing lines, compared to BE3 cells. Error bars represent s.e.m., n = 12 different target cytosines among 5 different sgRNAs, includes values from day 2 and day 6; asterisks (*) indicate a significant difference (p<0.05) between groups, using a one-way ANOVA with Tukey’s correction for multiple testing.
