Supplementary Figure 6: Expression of optimized base editors in stable NIH/3T3 and DLD1 cells
From: Optimized base editors enable efficient editing in cells, organoids and mice
a. Immunoblot showing editor expression from PGK-Puro and P2A-Puro vectors in NIH/3T3 cells. b. Immunoblot showing editor expression from PGK-Puro and P2A-Puro vectors in DLD1 cells. c. Relative mRNA abundance of RA, 2X, and FNLS editors in NIH/3T3 stable cell lines. Graphs show mean values. Error bars represent s.e.m., n = 3 biologically independent experiments; no significant differences (p<0.05) between any of the groups, using a one-way ANOVA with Tukey’s correction for multiple testing. d. Immunoblot showing expression of each optimized editor in NIH/3T3s, relative to Cas9. Each blot was repeated at least two times with similar results.
