Supplementary Figure 9: Additional free UGI further enhances base editing mediated by hA3A-BEs.
From: Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
(a) Statistical analysis of C-to-T fractions of base editing products induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D at cytosines of GpC sites in regions with natively low levels of DNA methylation shown in Figure 2h. n = 9 samples from three independent experiments. (b) Statistical analysis of normalized indel frequencies induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D in regions with natively low levels of DNA methylation shown in Figure 2i, setting the ones induced by hA3A-BE3-Y130F (left) or hA3A-BE3-Y132D (right) as 100%. n = 6 samples from three independent experiments. (c) Comparison of product purity yielded by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D in genomic DNA regions with natively high levels of DNA methylation. (d) Statistical analysis of C-to-T fractions of base editing products induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D shown in (c). n = 6 samples from three independent experiments. (e) Comparison of indels induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D in genomic DNA regions with natively high levels of DNA methylation. (f) Statistical analysis of normalized indel frequencies induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D in regions with natively high levels of DNA methylation shown in (e), setting the ones induced by hA3A-BE3-Y130F (left) or hA3A-BE3-Y132D (right) as 100%. n = 6 samples from three independent experiments. (g) Comparison of C-to-T editing frequencies induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D in genomic DNA regions with natively high levels of DNA methylation. (h) Statistical analysis of normalized C-to-T editing frequencies induced by hA3A-BE3-Y130F, hA3A-eBE-Y130F, hA3A-BE3-Y132D and hA3A-eBE-Y132D in regions with natively high levels of DNA methylation shown in (g), setting the ones induced by hA3A-BE3-Y130F (left) or hA3A-BE3-Y132D (right) as 100%. n = 6 samples from three independent experiments.
(a,b,d,f,h) P value, one-tailed Student’s t test. The median and IQR are shown.
(e,g) Means ± s.d. were from three independent experiments.
