Supplementary Figure 3: Comparison of base editing efficiencies induced by BE3 and hA3A-BE3 in genomic regions with induced high levels of DNA methylation.
From: Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
(a) Base editing efficiencies induced by BE3 and hA3A-BE3 in genomic regions with induced high levels of DNA methylation. The C-to-T editing frequencies of indicated cytosines were individually determined and target site sequences are shown with the BE3 editing window (position 4-8, setting the base distal to the PAM as position 1) in lavender, PAM in cyan and CpG site in capital. Means ± s.d. were from three independent experiments. (b) Statistical analysis of normalized C-to-T editing frequencies in regions with induced high levels of DNA methylation shown in (a), setting the ones induced by BE3 as 100%. n = 21 samples from three independent experiments. (c) Statistical analysis of normalized C-to-T editing frequencies at GpC sites in regions with natively or induced high levels of DNA methylation shown in (a) and Figure 1e, setting the ones induced by BE3 as 100%. n = 36 samples from three independent experiments.
(b,c) P value, one-tailed Student’s t test. The median and IQR are shown.
