Supplementary Figure 4: Comparison of base editing frequencies induced by BE3 and hA3A-BE3 in genomic regions with low levels of DNA methylation.
From: Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
(a) Base editing efficiencies induced by BE3 and hA3A-BE3 in genomic regions with natively low levels of DNA methylation. (b) Base editing efficiencies induced by BE3 and hA3A-BE3 in genomic regions with induced low levels of DNA methylation. (c) Statistical analysis of normalized C-to-T editing frequencies at C of GpC sites in regions with natively or induced low levels of DNA methylation shown in (a,b), setting the ones induced by BE3 as 100%. n = 39 samples from three independent experiments. (d) Statistical analysis of normalized C-to-T editing frequencies at non-CpG sites in regions with natively or induced low levels of DNA methylation shown in (a,b), setting the ones induced by BE3 as 100%. n = 24 samples from three independent experiments.
(a,b) Target site sequences are shown with the BE3 editing window (position 4-8, setting the base distal to the PAM as position 1) in lavender, PAM in cyan and CpG site in capital. Shaded gray, the guanines at 5’ end of editable cytosines. Means ± s.d. were from three independent experiments.
(c,d) P value, one-tailed Student’s t test. The median and IQR are shown.
