Supplementary Figure 10: eA3A-BE3 bearing VRQR or xCas9 mutations that alter PAM recognition specificity can be used to edit sites bearing NGA or NGT PAMs for highly precise single nucleotide editing.
From: An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities
Heat maps showing C-to-T editing efficiencies for VRQR BE3, eA3A-VRQR BE3, xCas9 BE3, and eA3A-xCas9 BE3 at 14 endogenous human gene target sites. VRQR target sites all bear NGAN PAM sequences, while xCas9 target sites bear NGN PAM sequences. Each site bears a cognate cytidine preceded by a 5′ T and one or more bystander cytidines within the editing window. Editing efficiencies shown represent the mean of three biological replicates. Graph shows cognate-to-bystander C to T editing ratios for VRQR BE3, eA3A-VRQR BE3, xCas9 BE3 and eA3A-xCas9 BE3. Data points shown represent ratios calculated from the mean values shown in the heat maps. Median values are indicated by a red line and error bars represent interquartile ranges. Values shown represent the mean of n = 3 biologically independent replicates.
