Supplementary Figure 12: Incorporating a self-cleaving hammerhead ribozyme on the 5′ end of the gRNA preserves perfect matching of the spacer and target site and rescues the activities of the eA3A-BE3 variants bearing HF1 and Hypa high-fidelity mutations.
From: An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities
Heat maps showing C-to-T editing efficiencies for eA3A variants incorporating HF1 or Hypa mutations targeting HBB -28 (A>G) using a gRNA with a 5′ mismatched guanine (top), a self-cleaving hammerhead ribozyme with 6 nucleotides of self-complementarity (middle), or a self-cleaving hammerhead ribozyme with 8 nucleotides of self-complementarity (bottom). The 5′ mismatched guanine or the ribozyme sequence is shown in red, while the spacer sequence is shown in black. Editing efficiencies shown represent the mean of three biological replicates.
