Supplementary Figure 4: Algorithm for zonation reconstruction based on the expression of hepatocytes landmark genes.
From: Paired-cell sequencing enables spatial gene expression mapping of liver endothelial cells
a-b) Zonation profiles set of 21 pericentral landmark genes (a) and 30 periportal genes (b). Profiles are taken from Bahar Halpern et al.9, each profile normalized by its maximum. c) The summed expression levels of the two landmark gene sets provide a convenient coordinate system to infer localization of cells. Shown are representative hepatocyte zonated genes from Bahar Halpern et al.9, each dot is a paired-cell, colors denote the genes’ expression levels, in units of log10(fraction of total UMIs). d) Gamma distribution fits for the frequencies of the scaled coordinate η in each layer (Online Methods). Distributions are based on the η values and the most likely layer from Bahar Halpern et al.9. Numbers above the curves denote the lobule layers.
