Supplementary Figure 6: Spatial sorting of LECs
From: Paired-cell sequencing enables spatial gene expression mapping of liver endothelial cells
a) Zonation of transcription factors34 and b) surface markers (GO:0009986) among LEC genes. Shown are zonated endothelial genes with a ratio of maximal to minimal zonation values larger than 1.5-fold, profiles are normalized by their maximum and sorted by centers of mass. Red box marks Kit, encoding CD117, which is used for the spatial sorting in c-d. c) FACS gating of CD31+CD45- LECs population. d) FACS sorting strategy of LEC spatial sorting by Kit, encoding the CD117 surface marker. Left panel shows the distribution of the CD117+ population out of CD31+ LECs. Four populations (P1-P4) were sorted according to CD117 levels. e) qPCR validation of the four sorted populations for selected genes. Blue - zonation profiles based on pcRNAseq, Red - qPCR results. All zonation profiles normalized to their mean across zones. Patches are s.e.m. n=4 mice. KruskalWallis p-values confirming the significance between the pericentral and periportal endothelial cells are shown at each plot title. Aqp1 is presened as a control of non-zonated LEC gene. FACS sorting and validations were performed on four mice.
