Supplementary Figure 7: Examples of tissues in which pcRNAseq could be readily applied to obtain spatial patterns.
From: Paired-cell sequencing enables spatial gene expression mapping of liver endothelial cells
a-d) Examples of cell populations that are interleaved with enterocytes within the small intestinal epithelium. a) Intra-epithelial lymphocytes express Cd3e (green dots, cells marked by white arrows). Red dots are mRNA for Slc2a2, an enterocyte marker. b) An enteroendocrine cell marked by a white arrow expresses Chga mRNA (green dots). c) Tuft cells express Dclk1 (green dots). White arrow marks a crypt cell, arrowhead marks a villus cell. Red dots are mRNA of Olfm4, expressed at the bottom of the intestinal crypts. d) Goblet cells, marked by white arrows, express Gob5 mRNA (green dots). Scale bars are 8μM. Gray in b-c is immunofluorescence for the cell surface marker E-cadherin. e) Hepatic Stellate cell expressing Acta2 mRNA (green dots, dashed yellow outline), adjacent to hepatocytes that express Acly (red dots). Blue in a-e is DAPI nuclear staining, scale bar 10 μM. f) Lymphocytes in a colonic tumor express Cd3e mRNA (red dots, cell marked with a white outline in the inset) and are adjacent to tumor cells expressing Nt5e (blue dots, yellow outline in the inset). Lgr5 mRNA (green dots) expressed in a distinct tumor cell subset. Scale bar 10 μM, inset scale bar 2 μM. In (a-f) micrographs are representative of 10 images with similar observations.
