Supplementary Figure 1: CD31 and CD45 enrich for distinct mouse liver single cell populations.
From: Paired-cell sequencing enables spatial gene expression mapping of liver endothelial cells
tSNE maps of FACS-sorted single NPCs, colored by the index-sorting values of CD31 (a) and CD45 (b). Only cells for which index sorting was obtained are shown (2,722 out of the 3,151 cells). Blue – high values, gray – low values. c) Scatter plot of CD31 and CD45 indices. Blue and red patches mark the CD31+CD45- and CD45+CD31- population analyzed in (d). d) CD31+CD45- cells have minimal contamination from other cell types. Shown are the summed UMI fraction of the cell-type specific markers for the seven cell types in Figure 2. Markers for non-activated hepatic stellate cells (Reln, Des, Gfap, Lrat) and for portal fibroblasts (Entpd2, Col15a1, Eln) were taken from61 (Thy1 was excluded as it is also a T cell marker). Boxes represent the mean of UMI fraction, n=3151 cells, Box plot elements: center line, median; box limits, first to third quartile (Q1 to Q3); whiskers, extend to the most extreme data point within 1.5× the interquartile range (IQR) from the box; circles, data points.
