Supplementary Figure 12: Effects of TRM1-10, three Flavobacterium strains, and P. putida KT2440 on the progress of bacterial wilt in Moneymaker.

Disease progress of bacterial wilt on Moneymaker treated with TRM1-10, F. aquidurense RC62, F. daejeonense RCH33, Flavobacterium sp. TCH3-2, or P. putida KT2440 using either (a) sterile commercial horticultural nursery soil or (b) non-sterile commercial horticultural nursery soil mixed with non-sterile natural field soil. One week prior to the inoculation of R. solanacearum SL341, tomato plants were treated with TRM1-10, each of the three Flavobacterium strains, or P. putida KT2440. Repeated measures ANOVA displayed a significant difference between TRM1-10 treatment and control treatment in sterile nursery soil (*, p = 0.01474) and in field soil mixed with non-sterile nursery soil (*, p = 0.01731). Treatment of each of the Flavobacterium strains was not significantly different from the non-treatment control for bacterial wilt suppression. Significant interaction between TRM1-10 treatment and experimental days were noticed for both cases: sterile nursery soil (p < 2.2e⁻¹⁶) and field soil mixed with non-sterile nursery soil (p = 4.013e⁻⁵). Treatment of P. putida KT2440 showed a significant difference as compared to the non-treated control only in sterile nursery soil (p = 0.0003345). Each vertical bar represents the standard error of the means from three replicates (each replicate with 10 plants, n = 30 in total for each treatment). Disease index: 0, no disease symptoms; 1, 1-25% of leaves wilted; 2, 26-50% of leaves wilted; 3, 51-75% of leaves wilted; 4, 76-100% of leaves wilted.