Supplementary Figure 13: Population dynamics and disease progress of TRM1-10 (TRM1) and R. solanacearum SL341 (Rsol) in tomato plants.

(a) Pearson correlation showing a strong positive correlation between colony count and qPCR throughout all experimental periods for TRM1-10 (left) and R. solanacearum SL341 (right) populations (n = 47 for TRM1, n = 28 for Rsol). (b) Population changes of TRM1-10 and R. solanacearum SL341 in bulk soil over time. Two-sample, two-sided t-tests were used to compare bacterial populations between samples (n = 5). The population of TRM1-10 remained stable in the bulk soil of Hawaii 7996. On the other hand, the population of TRM1-10 in bulk soil remarkably decreased in Moneymaker to almost undetectable levels. TRM1-10 populations differed between the two cultivars and were significantly higher in Hawaii 7996 than Moneymaker on D7 (p = 0.034), D10 (p = 3.25e⁻⁵), and D14 (p = 0.004). In Moneymaker, TRM1-10 showed significantly higher populations across all sample days, i.e. D7 (p = 0.039), D10 (p = 3.90e⁻⁴) and D14 (p = 0.003), treated with TRM1-10 and SL341 than TRM1-10 alone. The populations of SL341 decreased significantly (p = 5.32e⁻⁴) from D7 to D10 in TRM1+Rsol, but no significant difference was observed between D10 and D14 (p = 0.689). The populations of SL341 in Rsol alone and TRM1+Rsol showed no significant difference (p = 0.601) on D10; it was significantly higher in Rsol alone on D14 (p = 0.013). Each vertical bar represents the standard deviation of the mean from 5 plants. Asterisks indicate significant difference between treatments (*, p < 0.05; **, p < 0.01; ***, p < 0.001). (c) Bacterial wilt suppression by TRM1-10 and in planta population of SL341 in Moneymaker. Repeated measures ANOVA displayed a significant suppression of bacterial wilt in TRM1+Rsol than Rsol alone (*, p = 0.02635), a significant difference between experimental periods (p = 9.389e⁻⁹), and a significant interaction between treatments and experimental days (p = 2.572e⁻⁹). Each vertical bar represents the standard deviation of the mean from 14 plants remained until experimental period D14.