Supplementary Figure 3: Screen of Parkinson's disease–candidate genes for age-specific motor defects.

Animals were exposed to adult-only RNAi targeting 45 top candidate PD genes, and tested for thrashing defects on days 2, 5, and 8 of adulthood. Movement was analyzed using CeleST. (a) Heatmap of (hierarchically clustered) t-statistics comparing 10 CeleST movement measurements for each of the top 45 top PD gene candidates against the control L4440 RNAi on day 8 of adulthood, n ≥ 50 per gene (exact sample sizes per gene in Supplementary Data 13). (b) Pearson's correlation of t-statistics for each of the 10 CeleST movement measurements between all pairs of genes tested on days 2, 5, and 8 of adulthood. (c) Principal components were calculated using all 13,048 worms (across 45 genes and 3 days). PCA plot of RNAi-treated worms and control (aggregated by gene and day, see sample sizes in Supplementary Data 13). Colors indicate age of worm. PC1 (x-axis) and PC2 (y-axis) respectively account for 39.36% and 11.85% of the total variation. (d) Neuronal RNAi-sensitive animals were exposed to adult-only RNAi individually targeting 13 top cancer and metabolic disease predictions, bcat-1 (red) as a positive control, or the L4440 negative control. Curling was examined on day 8 using an automated analysis program (Sohrabi, et al. in preparation). Mean ± SEM. Control n=351, bcat-1 n=420, cyb-2.1 n=287, pxl-1 n=289, frm-2 n=279, mre-11 n=272, sma-4 n=286, snt-4 n=305, cdh-4 n=285, lbp-2 n=320, ani-3 n=300, hcp-1 n=264, BE0003N10.1 n=229, let-363 n=284, hil-3 n=270. n represents the number of animals per condition. One-way ANOVA with Tukey's multiple comparisons test. Control vs bcat-1i p= 4.33e-8. ****p<0.0001.