Supplementary Figure 9: Production of G-CSF using multiple InSCyT systems.

Dose size was 300 μg²⁴. Center points and error bars represent the mean and range, respectively, of technical triplicates unless otherwise noted. Fermentograms and UV profiles from chromatography operations on three InSCyT systems during G-CSF production. DO, dissolved oxygen; SLPM, standard liters per minute. Timeline and yields for production of G-CSF using three InSCyT systems. Wet cell weight (WCW) (black circles) and cumulative unpurified (orange) and formulated (blue) doses of G-CSF are shown. Grey circles show individual data points. Analysis of the tertiary structures of InSCyT G-CSF and a de-identified marketed product using circular dichroism (CD). Analysis of additional product-related variants. Black boxes represent the range of InSCyT G-CSF samples (Batches 1-6) with an additional line at the mean. SDS-PAGE (12% tris-glycine) analysis of samples from the USP during biomass accumulation (Perfusate Sample #0) and production (Perfusate Sample #1,2), and a final, formulated InSCyT sample (Formulated Sample #1,2) alongside a G-CSF marketed drug product (Std). M, molecular mass marker. Analysis of product purity by isoelectric focusing (IEF) for a formulated sample from each system. M, IEF protein marker. Example RPLC and SEC chromatograms used to quantify purity of G-CSF (representative of all six batches).