Supplementary Figure 5: The aminosilane surface is stable to Edman chemistry. | Nature Biotechnology

Supplementary Figure 5: The aminosilane surface is stable to Edman chemistry.

From: Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures

Supplementary Figure 5

(A) Schematic of assay. A layer of APTES was covalently formed on a glass surface by siloxane bond formation (-Si-O-Si-). (B) Counts of single Atto647N-NHS fluorophores (plotted as mean count, shown above, per microscopy field +/- s.d.), immobilized via a stable amide linkage to the silane layer, remain unchanged with repeated experimental cycles of Edman chemistry and after washes with wash buffer.

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