Supplementary Figure 7: Image-processing summary.
From: Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures
Fluorescent spots were identified using a Gaussian peak fitting algorithm, then images were aligned between experimental cycles based on positions of fiducial markers. Each spot’s position was extracted from the aligned images, with the spot categorized in each frame as either ON or OFF depending on the presence of a well-fitting peak. Spot intensities in each frame were then measured using Mexican hat photometry.
