Supplementary Figure 8: The sequence positions of multiple amino acid types can be determined by labeling each type with a distinct dye. | Nature Biotechnology

Supplementary Figure 8: The sequence positions of multiple amino acid types can be determined by labeling each type with a distinct dye.

From: Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures

Supplementary Figure 8

The histogram displays counts of individual molecules of the doubly labeled peptide GK‡AGAGAC♦AGAYG (summarizing data from 400 image fields) indicating the Edman cycle numbers at which the two dyes were removed. An example of an individual doubly-labeled molecule is shown in the extracted TIRF images at bottom right. ‡ indicates Janelia Fluor 549 conjugated to lysine and ♦indicates Atto647N conjugated to cysteine.

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