Supplementary Figure 13: Full one- and two-dye fluorosequencing histograms for peptides in Fig. 5a and Fig. 5d confirm signal for the expected sequence patterns.
From: Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures
(A) Histograms tallying counts of molecules sequenced from a mixture of GC♦AGC♦AGAG with GAGC♦GAC♦GAGAD (left panel, 98 fields) and GAC♦C♦AGAAD with GAGC♦GAC♦GAGAD (right panel, 49 fields) (B) Histograms of sequenced peptide RK†TTRK†M, 49 fields. ♦ indicates Atto647N conjugated to cysteine and †indicates Atto647N coupled to lysine residues.
