Supplementary Figure 14: One-, two-, and three-dye fluorosequencing histograms for insulin peptides (Fig. 5b) confirm signal for the expected sequence patterns.
From: Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures

Plotted are the background-adjusted one, two, and three dye histograms for insulin A2 chain (QC♦C♦TSIC♦SLYNE, measured across 100 image fields), A3 chain (NYC♦N, 120 fields), B1 chain (FVNQHLC♦GSHLVE, 72 fields), and B2 chain (ALYLVC♦GE, 100 fields), respectively. ♦ indicates Atto647N conjugated to cysteine.