Supplementary Figure 1: Only select fluorophores exhibit fluorescence stability toward Edman reagents.
From: Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures
(A) Fluorophores (spanning four fluorescent channels, denoted by bar colors) were tested for their percentage change in fluorescence intensity in PBS buffer, following a 24 hour incubation with TFA or pyridine/PITC (shown as pyridine). Dyes marked with boxes exhibited only moderate changes (<20%) in fluorescence. The data are presented as mean across replicates, shown, with error +/- s.d. where n ≥ 3. (B) Images of fluorophore coupled Tentagel beads illustrate fluorescence changes by Edman reagents. In the case of BODIPY-FL (left panels), the fluorescence intensity decreases with TFA incubation, while there is a spectral redshift with pyridine incubation. In contrast, Atto647N (right panels) is stable in both color and intensity to both conditions. Scale bar, 200μm.
