Supplementary Figure 8: Blocking reagents reduce nonspecific probe binding for flow cytometry.
From: ClampFISH detects individual nucleic acid molecules using click chemistry–based amplification
MDA-MB 231 cells expressing GFP were mixed with MDA-MB 231 cells not expressing GFP at 50%. The mixed cell population was subsequently stained with clampFISH probes targeting GFP mRNA and the separation was assessed with the addition of different blocking reagents in the hybridization buffer.
