Supplementary Figure 13: Proximity dependence of clampFISH arms.
From: ClampFISH detects individual nucleic acid molecules using click chemistry–based amplification
(a) Fluorescent signal from clampFISH probes targeting GFP mRNA on WM983bcells expressing GFP. Arms are hybridized adjacent to each other and clicked (bottom) Fluorescent signal remaining on the same cells after stripping with 60% formamide. (b) Fluorescent signal from clampFISH probes targeting GFP mRNA on WM983bcells expressing GFP. Arms are adjacent to each other without clicking (bottom) Fluorescent signal remaining on the same cells after stripping with 60% formamide. (c) Fluorescent signal from clampFISH probes targeting GFP mRNA on WM983bcells expressing GFP. One arm is specific to the GFP target while the other is non-targeting and the sample is clicked (bottom) Fluorescent signal remaining on the same cells after stripping with 60% formamide. (d) Fluorescent signal from clampFISH probes targeting GFP mRNA on WM983b cells that are not expressing GFP. Arms are hybridized adjacent to each other and clicked (bottom) Fluorescent signal remaining on the same cells after stripping with 60% formamide.
