Supplementary Figure 2: Sensitivity and selectivity assessment.
From: ClampFISH detects individual nucleic acid molecules using click chemistry–based amplification
(a) Fraction of clampFISH spots that colocalize with single molecule RNA FISH spots (blue) and spots that do not colocalize with single molecule RNA FISH spots (red) as the threshold for spot calling is relaxed. (b) (blue) Fraction of all clampFISH spots that colocalize with single molecule RNA FISH spots as the threshold is relaxed, (green) fraction of all single molecule RNA FISH spots that colocalize with clampFISH spots. Note: Single-molecule spot localization is difficult at very high fluorescence levels, making stringent spot localization difficult for bright clampFISH spots. This may contribute to an apparent (false) loss of colocalization with single molecule RNA FISH spots, whose location is easier to computationally estimate.
