Supplementary Figure 5: Estimation of whole genome ANI from fragmented genomes.

To evaluate the impact of genome fragmentation on whole-genome average nucleotide identity (wgANI) estimation, pairs of genomes from NCBI RefSeq with wgANI ≥ 70% and ≥ 20kb were selected, random fragments were generated (from 1 to 45kb) from one of the two genomes, and then compared to the other complete genome. The resulting estimated wgANI between the fragment and complete genome was then compared with the original values estimated from the two complete genomes (y-axis). Boxplots lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles), while whisker extend from the nearest hinge to the smallest/largest value no further than 1.5 * IQR from the hinge (where IQR is the inter-quartile range, or distance between the first and third quartiles).