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Conditional alleles, inteins and degrons

Nature Biotechnology volume 23, page 18 (2005)Cite this article

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To the editor:

In a Letter in the July issue (Nat. Biotechnol. 22, 871–876, 2004), Martin Zeidler et al. describe a new approach for generating temperature-sensitive alleles based on conditionally active inteins. The system described is undoubtedly a novel and valuable alternative to 'classical' temperature-sensitive alleles. The authors of the Letter, as well as Francine Perler in an accompanying News and Views article (Nat. Biotechnol. 22, 824–826, 2004), discuss in detail the advantages of the intein-based system over classical temperature-sensitive alleles. However, both Zeidler et al. and Perler neglect to mention that one alternative to the classical temperature-sensitive allele has already been described and successfully used.

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  1. The Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, Vienna, A-1030, Austria

    Juraj Gregan

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  1. Juraj Gregan
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Gregan, J. Conditional alleles, inteins and degrons. Nat Biotechnol 23, 18 (2005). https://doi.org/10.1038/nbt0105-18a

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