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Interspecific Plant Hybridization by Electrofusion in Nicotiana

Bio/Technology volume 4, pages 57–60 (1986)Cite this article

Abstract

Mesophyll protoplasts from Nicotiana glauca and Nicotiana langsdorffii were fused electrically. From a total of 9 × 104 electrically fused protoplasts (4.5 × 104 each), we obtained more than 96 green hybrid colonies that grew vigorously on hormone–free medium after 10 weeks of culture. Electrofocusing analysis of Fraction I proteins isolated from hybrid calluses confirmed the hybridity of the calluses. The hybrid isolation yield was about 0.1 percent. All hybrid colonies regenerated shoots after about 4 months of culture and some formed roots.

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Authors and Affiliations

  1. Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Kyoto, 606, Japan

    Hiromichi Morikawa, Katsuhiko Sugino, Yasuyuki Hayashi & Yasuyuki Yamada

  2. Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto, 606, Japan

    Junko Takeda & Mitsugi Senda

  3. Graduate Division of Biochemical Regulation, Faculty of Agriculture, Nagoya University, Nagoya, 464, Japan

    Atsushi Hirai

Authors
  1. Hiromichi Morikawa
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  2. Katsuhiko Sugino
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  3. Yasuyuki Hayashi
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  4. Junko Takeda
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  5. Mitsugi Senda
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  6. Atsushi Hirai
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  7. Yasuyuki Yamada
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Morikawa, H., Sugino, K., Hayashi, Y. et al. Interspecific Plant Hybridization by Electrofusion in Nicotiana. Nat Biotechnol 4, 57–60 (1986). https://doi.org/10.1038/nbt0186-57

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  • DOI: https://doi.org/10.1038/nbt0186-57

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