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Enzymes by post—restriction enzyme stability

Nature Biotechnology volume 18, page 243 (2000)Cite this article

To the editor:

DNA restriction enzymes, with their widespread applications in many diagnostic procedures (e.g., RFLP, PCR, etc), are the very cornerstone of recombinant DNA technology. Unfortunately, their transport and storage are usually carried out at subzero temperatures, greatly increasing the cost per unit activity and limiting availability in many developing countries.

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Figure 1: Ethidium bromide-stained agarose gels of restriction digests of 0.5 μg λ-DNA, using 1 μl of serially diluted restriction enzymes (in supplied 1× buffer).

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  1. Moredun Research Institute, Pentlands, Science Park, Bush Loan, Penicuik, EH25 0PS, Scotland, UK

    John B. March & Jason Clark

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  1. John B. March
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  2. Jason Clark
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March, J., Clark, J. Enzymes by post—restriction enzyme stability. Nat Biotechnol 18, 243 (2000). https://doi.org/10.1038/73590

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