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The α-Amylase Gene as a Marker for Gene Cloning: Direct Screening of Recombinant Clones

Bio/Technology volume 8, pages 241–242 (1990)Cite this article

Abstract

We report the construction and use of a new system for the direct screening of recombinant clones after transformation. The system uses a Bacillus subtilis-Escherichia coli shuttle vector that carries the B. subtilis structural gene for α-amylase. Insertion of foreign DNA into this gene results in a loss of amylolytic activity in the host cells that can be assayed using a simple and inexpensive staining procedure.

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Author notes

  1. Ana Clara G. Schenberg: Corresponding author.

Authors and Affiliations

  1. Genetics Institute, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Caixa Postal 83, Piracicaba, CEP 13400, SP, Brazil

    Nilo Ikuta & Aline Pizzirani-Kleiner

  2. Department of Cell Biology, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, CEP 70000, DF, Brazil

    Maria Beatriz N. Souza, Fernando F. Valencia, Maria Elita B. Castro & Spartaco Astolfi-Filho

  3. Department of Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, Cidade Universitária, São Paulo, CEP 05508, SP, Brazil

    Ana Clara G. Schenberg

Authors
  1. Nilo Ikuta
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  2. Maria Beatriz N. Souza
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  3. Fernando F. Valencia
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  4. Maria Elita B. Castro
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  5. Ana Clara G. Schenberg
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  6. Aline Pizzirani-Kleiner
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  7. Spartaco Astolfi-Filho
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Ikuta, N., Souza, M., Valencia, F. et al. The α-Amylase Gene as a Marker for Gene Cloning: Direct Screening of Recombinant Clones. Nat Biotechnol 8, 241–242 (1990). https://doi.org/10.1038/nbt0390-241

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  • DOI: https://doi.org/10.1038/nbt0390-241

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