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Production of High Solids Tomatoes Through Molecular Modification of Levels of the Plant Growth Regulator Cytokinin

Bio/Technology volume 13, pages 250–254 (1995)Cite this article

Abstract

Chimeric isopentenyl transferase (ipt) gene constructs were prepared and introduced into tomato plants via Agrobacterium-mediated transformation. Expression of the ipt gene, which encodes a key enzyme involved in the biosynthesis of the plant growth regulator cytokinin, was modulated using a promoter from a gene expressed primarily in tomato ovaries. As expected, the ipt gene was expressed, and levels of cytokinin were increased, in ovaries of the transgenic plants. Plant yield and fruit processing characteristics of these transgenic plants were examined during three consecutive years of field testing. Levels of total solids were significantly increased in six of seven, and soluble solids were significantly increased hi five of seven, independent transgenic tomato lines.

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References

  1. Rick, C.M. 1974. High soluble-solids content in large-fruited tomato lines from a wild green-fruited species. Hilgardia 42: 493–510.

    Article  Google Scholar 

  2. Davies, J.N. and Hobson, G.E. 1981. The constituents of tomato fruit—the influence of environment, nutrition and genotype. CRC Crit. Rev. Food Sci. Nutr. 15: 205–280.

    Article  CAS  Google Scholar 

  3. Stevens, M.A., Kader, A.A. and Albright-Holton, M. 1997. Intereultivar variation in composition of locular and pericarp portions of fresh market tomatoes. J. Amer. Soc. Hort. Sci. 102: 689–692.

    Google Scholar 

  4. Hewitt, J.D., Dinar, M. and Stevens, M.A. 1982. Sink strength of fruits of two tomato genotypes differing in total fruit solids content. J. Amer. Soc. Hort. Sci. 107: 896–900.

    CAS  Google Scholar 

  5. Gersani, M. and Lips, S.H. 1980. The influence of shoots, roots, and hormones on sucrose distribution. J. Expt. Biol. 31: 177–184.

    CAS  Google Scholar 

  6. Leonard, M., Kinet, J.-M., Bodson, M. and Bernier, G. 1983. Enhanced inflorescence development in tomato by growth substance treatments in relation to 14C-assimilate distribution. Physiol. Plant. 57: 85–89.

    Article  CAS  Google Scholar 

  7. Thomas, T.H. 1985. Hormonal control of assimilate movement and compart-mentation, p. 350–359. In: Plant Growth Substances. Bopp, M. (Ed.). Springer-Verlag Berlin.

    Google Scholar 

  8. Lenton, J.R. 1984. Are plant growth substances involved in the partitioning of assimilate to developing reproductive sinks? Plant Growth Regulation 2: 267–276.

    Article  CAS  Google Scholar 

  9. Martineau, B. and Houck, C.M. 1992. Ovary-tissue transcriptional factors. U.S. patent no. 5,175,095.

  10. Martineau, B., McBride, K.E. and Houck, C.M. 1991. Regulation of metallo-carboxypeptidase inhibitor gene expression in tomato. Mol. Gen. Genet. 228: 281–286.

    Article  CAS  Google Scholar 

  11. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

  12. Martineau, B., Houck, C.M., Sheehy, R.E. and Hiatt, W.R. 1994. Fruit-specific expression of the A. tumefaciens isopentenyl transferase gene in tomato: effects on fruit ripening and defense-related gene expression in leaves. Plant J. 5: 11–19.

    Article  CAS  Google Scholar 

  13. Barker, R.F., Idler, K.B., Thompson, D.V. and Kemp, J.D. 1983. Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol. Biol. 2: 335–350.

    Article  CAS  Google Scholar 

  14. McBride, K.E. and Summerfelt, K.R. 1990. Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 14: 269–276.

    Article  CAS  Google Scholar 

  15. Redenbaugh, K., Hiatt, W., Martineau, B., Kramer, M., Sheehy, R., Sanders, R., Houck, C. and Emlay, D. 1992. Safety assessment of genetically engineered fruits and vegetables: A case study of the Flavr Savr™ Tomato. CRC Press, Boca Raton.

  16. Crosby, K.E., Aung, L.H. and Buss, G.R. 1981. Influence of 6-benzylamino purine on fruit-set and seed development in two soybean, Glycine max (L.) Merr. genotypes. Plant Physiol. 68: 985–988.

    Article  CAS  Google Scholar 

  17. Carlson, D.R., Dyer, D.J., Cotterman, C.D. and Durley, R.C. 1987. The physiological basis for cytokinin induced increases in pod set in IX93-100 soybean. Plant Physiol. 84: 233–239.

    Article  CAS  Google Scholar 

  18. Peterson, C.M., Williams, J.C. and Kuang, A. 1990. Increased pod set of determinate cultivars of soybean, Glycine max, with 6-benzylaminopurine. Botanical Gazette 151: 322–330.

    Article  CAS  Google Scholar 

  19. Rylott, P.D. and Smith, M.L. 1990. Effects of applied plant growth substances on pod set in broad beans (Vicia faba var. major). J. Agric. Sci., Cambridge 114: 41–47.

    Article  CAS  Google Scholar 

  20. Atkins, C.A. and Pigeaire, A. 1993. Application of cytokinins to flowers to increase pod set in Lupinus angustifolius L. Aust. J. Agric. Res. 44: 1799–1819.

    Article  CAS  Google Scholar 

  21. Ho, L.C. 1984. Partitioning of assimilates in fruiting tomato plants. Plant Growth Regulation 2: 277–285.

    Article  CAS  Google Scholar 

  22. Ho, L.C. and Hewitt, J.D. 1986. Fruit development, p. 201–239. In: Tomato Crop. Atherton, J. G. and Rudich, J. (Eds.). Chapman and Hall, New York.

    Chapter  Google Scholar 

  23. Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J. and Schilperoort, R.A. 1983. A binary vector strategy based on separation of vir and T-region of the Agrobacterium tumefaciens Ti plasmid. Nature 303: 179–181.

    Article  CAS  Google Scholar 

  24. Holsters, M., DeWaele D., Depicker, A., Messens, E., Van Montagu, M. and Schell, J. 1978. Transfection and transformation of Agrobacterium tumefaciens. Mol. Gen. Genet. 163: 181–187.

    Article  CAS  Google Scholar 

  25. Fillatti, J.J., Kiser, J., Rose, R. and Comai, L. 1987. Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Bio/Techology 5: 726–730.

    CAS  Google Scholar 

  26. Sanders, R.A., Sheehy, R.E. and Martineau, B. An efficient and reliable method for determining the number of transgenes inserted into transgenic plants. Plant Mol. Biol. Rep. 10: 164–172.

    Article  CAS  Google Scholar 

  27. Steele, R.G.D. and Torre, J.H. 1980. Principals and Procedures of Statistics: A Biometrical Approach (2nd Ed.). McGraw-Hill, New York.

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Author information

Authors and Affiliations

  1. Calgene, Inc., 1920 Fifth Street, 28605, County Road 104, Davis, CA, 95616

    Belinda Martineau & Kristin R. Summerfelt

  2. Campbell Institute for Research and Technology, 28605, County Road 104, Davis, CA, 95616

    Dawn F. Adams & Joseph W. DeVerna

Authors
  1. Belinda Martineau
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  2. Kristin R. Summerfelt
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  3. Dawn F. Adams
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  4. Joseph W. DeVerna
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Correspondence to Belinda Martineau.

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Martineau, B., Summerfelt, K., Adams, D. et al. Production of High Solids Tomatoes Through Molecular Modification of Levels of the Plant Growth Regulator Cytokinin. Nat Biotechnol 13, 250–254 (1995). https://doi.org/10.1038/nbt0395-250

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