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Cloning efficiency and differentiation

Nature Biotechnology volume 19, page 406 (2001)Cite this article

To the editor

The efficiency of nuclear transfer remains extremely low (<2%) and high rates of perinatal mortality are observed, particularly when adult somatic cells are used as donor karyoplasts. This inefficiency hinders broader application of the procedure to biotechnology applications. Whereas cell cycle status of donor cells, incomplete reprogramming of donor nuclei, telomere length of cloned embryos, mitochondrial DNA (mtDNA) status of the recipient oocyte, X-chromosome inactivation, and DNA methylation or gene imprinting all influence cloning efficiency, I believe another factor is critical in determining success.

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Authors and Affiliations

  1. Laboratory for Reproductive Medicine Brown University and Women & Infants Hospital Marine Biological Laboratory, Woods Hole, 02543, MA

    Lin Liu

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  1. Lin Liu
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Liu, L. Cloning efficiency and differentiation. Nat Biotechnol 19, 406 (2001). https://doi.org/10.1038/88052

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