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Antisense RNA Inhibition of β-Glucuronidase Gene Expression in Transgenic Tobacco can be Transiently Overcome Using a Heat-Inducible β-Glucuronidase Gene Construct

Bio/Technology volume 8, pages 459–464 (1990)Cite this article

Abstract

A transgenic tobacco plant in which the constitutive expression of a single E. coli β-glucuronidase (GUS) gene had been completely repressed by antisense GUS RNA was crossed with another tobacco transformant containing several copies of the GUS gene driven by a 1.2 Kb D. melanogaster hsp70 promoter fragment. Southern blot analysis of the segregants in the F1 generation revealed different combinations of the sense, antisense and heat-inducible chimaeric GUS gene constructs. GUS activity in these segregants correlated with the presence and absence of the different GUS gene constructs. Transient heat induction of GUS activity was observed in some segregants in spite of the presence of the constitutive antisense GUS DNA construct. It was also possible to demonstrate the presence of sense GUS mRNA concomitant with the heat-inducible restoration of GUS activity in a plant where antisense GUS RNA had effectively suppressed GUS expression.

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Author notes

  1. Laurian S. Robert

    Present address: Plant Research Centre, Agr. Can. Research Branch, K.W. Neatby Bldg, C.E.F., Ottawa, Ont., Canada, K1A 0C6

  2. Steven F. Fabijanski: Corresponding author.

Authors and Affiliations

  1. Department of Biochemistry, University of Ottawa, 40 Marie Curie, Ottawa, Ont., Canada, K1N 6N5

    Pauline A. Donaldson, Christine Ladaique & Illimar Altosaar

  2. Paladin Hybrids Inc., P.O. Box 219, Brampton, Ont., Canada, L6V 2L2

    Paul G. Arnison & Steven F. Fabijanski

Authors
  1. Laurian S. Robert
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  2. Pauline A. Donaldson
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  3. Christine Ladaique
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  4. Illimar Altosaar
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  5. Paul G. Arnison
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  6. Steven F. Fabijanski
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Robert, L., Donaldson, P., Ladaique, C. et al. Antisense RNA Inhibition of β-Glucuronidase Gene Expression in Transgenic Tobacco can be Transiently Overcome Using a Heat-Inducible β-Glucuronidase Gene Construct. Nat Biotechnol 8, 459–464 (1990). https://doi.org/10.1038/nbt0590-459

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  • DOI: https://doi.org/10.1038/nbt0590-459

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