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Field tolerance to fungal pathogens of Brassica napus constitutively expressing a chimeric chitinase gene

Nature Biotechnology volume 14, pages 643–646 (1996)Cite this article

Abstract

Constitutive overexpression of a protein involved in plant defense mechanisms to disease is one of the strategies proposed to increase plant tolerance to fungal pathogens. A hybrid endochitinase gene under a constitutive promoter was introduced by Agrobacterium-mediated transformation into a winter-type oilseed rape (Brassica napus var. oleifera) inbred line. Progeny from transformed plants was challenged using three different fungal pathogens (Cylindrosporium concentricum, Phoma lingam, Sclerotinia sclerotiorum) in field trials at two different geographical locations. These plants exhibited an increased tolerance to disease as compared with the nontransgenic parental plants.

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Author notes

  1. Alain Toppan: e-mail: atoppan@mail.starnet.unisoft.fr.

Authors and Affiliations

  1. Unité de Phytotechnologie, Sanofi Recherche, Centre de Labège, F-31676, Labège Cedex, France

    René Grison, Bruno Grezes-Besset, Michel Schneider, Nicole Lucante, Luellen Olsen, Jean-Jacques Leguay & Alain Toppan

  2. Rustica Prograin Génétique, Domaine de Sandreau, F-31700, Mondonville, France

    René Grison, Bruno Grezes-Besset, Nicole Lucante & Alain Toppan

  3. Institut de Biologie Végétale, Université de Fribourg, 3 rue Albert Gockel, CH-1700, Fribourg, Switzerland

    Michel Schneider

  4. Service Développement Biotechnologie, Sanofi Recherche, Centre de Labège, F-31676, Labège Cedex, France

    Luellen Olsen

  5. Commissariat à l'Energie Atomique, Centre de Cadarache, F-13108, Saint Paul lez Durance Cedex, France

    Jean-Jacques Leguay

Authors
  1. René Grison
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  2. Bruno Grezes-Besset
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  3. Michel Schneider
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  4. Nicole Lucante
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  5. Luellen Olsen
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  6. Jean-Jacques Leguay
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  7. Alain Toppan
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Grison, R., Grezes-Besset, B., Schneider, M. et al. Field tolerance to fungal pathogens of Brassica napus constitutively expressing a chimeric chitinase gene. Nat Biotechnol 14, 643–646 (1996). https://doi.org/10.1038/nbt0596-643

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  • DOI: https://doi.org/10.1038/nbt0596-643

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