In vitro protein synthesis using a mixture of purified components now offers the possibility of efficient production of labile products and more detailed study of the translation process.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
An integrated cell‐free metabolic platform for protein production and synthetic biology
Molecular Systems Biology Open Access 14 October 2008
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
References
Shimizu et al. Nat. Biotechnol. 19, 751–755 (2001).
Ehrenberg, M. et al. J. Mol. Biol 211, 739–749 (1990).
Bremmer, H. & Dennis, P.P. in Escherichia coli and Salmonella: Cellular and Molecular Biology (eds. Neidhardt, F.C. et al.) 1553–1569 (ASM Press, Washington, DC, 1996).
Pavlov, M.Y. and Ehrenberg, M. ARchives Biochem.Biophys. 328, 9–16 (1996).
Kim, D.-M. et al. Eur. J. Biochem. 239, 881–886 (1996).
Kim, D.-M. & Swartz, J.R. Biotechnol. Bioeng. 71, 309–316 (2001).
Kim, D.-M. & Swartz, J.R. Biotechnol. Prog. 16, 385–390 (2000).
Schaffitzel, C. et al. J. Immunol. Meth. 231, 119–135 (1999).
Author information
Authors and Affiliations
Additional information
The online version of the original article can be found at 10.1038/90802
Rights and permissions
About this article
Cite this article
Swartz, J. A PURE approach to constructive biology. Nat Biotechnol 19, 732–733 (2001). https://doi.org/10.1038/90773
Published:
Issue date:
DOI: https://doi.org/10.1038/90773
This article is cited by
-
An integrated cell‐free metabolic platform for protein production and synthetic biology
Molecular Systems Biology (2008)
