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Applications of display technology in protein analysis

Nature Biotechnology volume 18, pages 1251–1256 (2000)Cite this article

Abstract

Display technology refers to a collection of methods for creating libraries of modularly coded biomolecules that can be screened for desired properties. It has become a routine tool for enriching molecular diversity and producing novel types of proteins. The combination of an ever-increasing variety of libraries of modularly coded protein complexxes with the development of innovative approaches to select a wide array of desired properties has facilitated large-scale analyses of protein–protein/protein–substrate interactions, rapid isolation of antibodies (or antibody mimetics) without immunization, and function-based protein analysis. Several practical and theoretical challenges remain to be addressed before display technology can be readily applied to proteomic studies.

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Figure 1
Figure 2: Examples of four major display systems.

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Acknowledgements

I thank William J. Dower for many insightful discussions and Rachel Derin for assistance in manuscript preparation. The work in the author's laboratory is supported by grants from National Institute of Health.

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  1. Department of Physiology and Department of Neuroscience The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, 21205, MD

    Min Li

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Li, M. Applications of display technology in protein analysis. Nat Biotechnol 18, 1251–1256 (2000). https://doi.org/10.1038/82355

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