Figure 1: Downregulation of ZEB1 expression by shRNA or siRNAs in MCF12A control and p53R175H mutant cells.
From: p53 regulates epithelial–mesenchymal transition and stem cell properties through modulating miRNAs
(a) MCF12A cells expressing p53R175H mutant were infected with lentivirus encoding control shRNA or ZEB1 shRNA (clone ID: TRCN0000017566, Open Biosystems). After puromycine selection for 10 days, infected cells were subjected to immunoblotting analysis (ZEB1 (D80D3) antibody, #3396, Cell signalling; 1:1000). Immunoblot shows the expression of ZEB1 from MCF12A control and infected p53R175H cells. (b) MCF12A cells expressing p53R175H were transfected with two individual ZEB1 siRNAs (ZEB1-1, lane2, SASI_Hs02_00330526, Sigma; ZEB1-2, SASI_Hs02_00330527, Sigma). At 48-h post-transfection, transfected cells were subjected to immunoblotting analysis.
