Supplementary Figure 4: A role of TAp73 in anti-oxidant defense.
From: TAp73 enhances the pentose phosphate pathway and supports cell proliferation
(a) TAp73+/+ and TAp73−/− MEF cells treated with control or G6pd siRNA. ROS was measured by 2′, 7′-di-chlorofluorescein (DCF) staining and flow cytometry (left), and protein expression by Western blot (Right). Western blots represent three independent experiments. (b) ROS levels in TAp73+/+ and TAp73−/− MEF cells that were treated with or without DHEA. (c) IMR90 cells were transfected with p73, G6PD, and control siRNA as indicated. ROS was measured. (d) TAp73+/+ and TAp73−/− MEF cells were treated with or without 50 mM H2O2 for 30 min and then cultured for 24 h in the presence or absence of DHEA. Cell viability was analyzed. Data are means ± SD (n = 3 independent experiments). (e) U2OS cells were transfected with control siRNA (−), p73 siRNA, and G6PD siRNA as indicated. Cells were treated with or without 250 μM H2O2 for 24 h and cell viability was analyzed by trypan blue staining. Data are means ± SD (n = 3 independent experiments).
