Supplementary Figure 5: p73 regulates DNA synthesis and cell senescence via G6PD.
From: TAp73 enhances the pentose phosphate pathway and supports cell proliferation
(a,b) p53−/− and p21−/− HCT116 cells were transfected with control (−), p73, and G6PD siRNA as indicated. Cells were assayed for BrdU incorporation. Data are means ± SD (n = 3 independent experiments). (c,d) Cell-cycle profile of p53−/− (c) and p21−/− (d) HCT116 cells transfected with p73, G6PD, and control siRNA as indicated. Protein expression is shown below. Western blots represent three independent experiments. (e) Percentage of SA- β-gal positive cells in IMR90 culture that were transfected with control, p73, or G6PD siRNA, and cultured for 72 h. Protein expression is shown below. Data are means ± SD (n = 3 independent experiments). Western blots represent three independent experiments.
