Supplementary Figure 6: p73 promotes cell proliferation through G6PD.
From: TAp73 enhances the pentose phosphate pathway and supports cell proliferation
(a–c) Proliferation of p53+/+ (a), p53−/− (b), and p21−/− (c) HCT116 cells transfected with the control, G6PD, and p73 siRNAs as indicated. Data are means ± SD (n = 3 independent experiments). (d) Growth of U2OS cells transfected with control siRNA or p73 siRNA, plus control vector or Flag-G6PD. Data are means ± SD (n = 3 independent experiments). Protein expression is shown on the Right. Western blots represent three independent experiments. (e–h) Growth of TAp73+/+ and TAp73−/− MEF cells stably overexpressing G6PD or vector control. Cells were cultured in medium containing vehicle (e), NAC (f), four ribonucleosides and four deoxyribonucleosides (Nuc) (g), or both NAC and Nuc (h). Data are means ± SD (n = 3 independent experiments).
