Supplementary Figure 3: E-Cadherin localizes across the medioapical surface in diaM mutants. | Nature Cell Biology

Supplementary Figure 3: E-Cadherin localizes across the medioapical surface in diaM mutants.

From: Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction

Supplementary Figure 3

(a,b) Serial z-sections of confocal images from two different fixed diaM embryos expressing Myo::GFP and stained for E-Cadherin. Medioapical E-Cadherin appears to be associated with the cortex and is localized in the same plane as Myo-II (arrows). Medioapical E-Cadherin in diaM mutants can be distinguished from subapical E-Cadherin puncta that are likely to represent endocytic vesicles. Distances indicated are positions relative to the apical z-slice (0 m). Scale bars are 5 μm.

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