Supplementary Figure 1: Venus::Rok(K116A) localizes to medioapical foci during constriction.

During apical constriction, Venus::Rok(K116A) accumulates in medioapical foci in ventral furrow cells, similar to Venus::Rok(WT). Images are from a live embryo expressing Gap43::ChFP (subapical slice) and Venus::Rok(K116A) (apical projection). Apical constriction appears to be unaffected by expression of Rok(K116A). Scale bar is 5 μm.